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pENTR4-RBPMS
(Plasmid #59387)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59387 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR 4
  • Backbone manufacturer
    Life Technologies, Modified by Tuschl Lab
  • Backbone size w/o insert (bp) 2279
  • Total vector size (bp) 2870

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RBPMS
  • Alt name
    RNA binding protein with multiple splicing
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    591
  • GenBank ID
    NM_001008710 513126872
  • Entrez Gene
    RBPMS (a.k.a. HERMES)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR4-RBPMS was a gift from Thomas Tuschl (Addgene plasmid # 59387 ; http://n2t.net/addgene:59387 ; RRID:Addgene_59387)

  • For your References section:

    Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets. Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, Larsson E, Ohler U, Tuschl T. RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014 May 23. 10.1261/rna.045005.114 PubMed 24860013
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