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Addgene

pET-OleD
(Plasmid #59373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OleD
  • Species
    Streptomyces antibioticus
  • Insert Size (bp)
    1314
  • GenBank ID
    DQ195536
  • Promoter T7 promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGTCCGGCGTAGAGGATCG
  • 3′ sequencing primer GGGCCTCTTGCGGGATATC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-OleD was a gift from Christopher Anderson (Addgene plasmid # 59373 ; http://n2t.net/addgene:59373 ; RRID:Addgene_59373)
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