pBAD-OleD
(Plasmid
#59372)
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PurposeInducible expression of OleD (DQ195536) under arabinose promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 59372 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNE2001
- Backbone size w/o insert (bp) 3975
- Total vector size (bp) 5307
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOleD-His
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SpeciesStreptomyces antibioticus
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Insert Size (bp)1332
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GenBank IDDQ195536
- Promoter pBAD
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TTTTTATCCATAAGATTAGCGGATCC
- 3′ sequencing primer CAGACCGCTTCTGCGTTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-OleD was a gift from Christopher Anderson (Addgene plasmid # 59372 ; http://n2t.net/addgene:59372 ; RRID:Addgene_59372)