pICE-FLAG-NAT10-siR-G641E
(Plasmid #59366)

• Purpose: Plasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
• Depositing Lab: Steve Jackson
• Publication: Larrieu et al Science. 2014 May 2;344(6183):527-32. doi: 10.1126/science.1252651.

Backbone

• Vector backbone: pICE
• Backbone size w/o insert (bp): 5044
• Total vector size (bp): 8086
• Modifications to backbone: FLAG tag inserted between HindIII and BamHI of original pICE
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NAT10 NM_024662
• Species: H. sapiens (human)
• Mutation: G641E and silent mutations to render the cDNA resistant to siRNA
• GenBank ID:
• Entrez Gene: NAT10 (a.k.a. ALP, Kre33, NET43)
• Promoter: CMV-tet
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: CMVpromoter forward
• 3′ sequencing primer: BGHpolyA reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry