pNICE HA-mSYD1B
(Plasmid #59362)

• Purpose: Expression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)
• Depositing Lab: Peter Scheiffele
• Publication: Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi: 10.1016/j.neuron.2013.05.010.

Backbone

• Vector backbone: pNICE-HA-N
• Backbone size w/o insert (bp): 3984
• Total vector size (bp): 8038
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mSYD1B
• Alt name: Syde2
• Alt name: synapse defective 1, Rho GTPase, homolog 2 (C. elegans)
• Alt name: mammalian SYD1B
• Species: M. musculus (mouse)
• Insert Size (bp): 4054
• GenBank ID: NM_001166064
• Entrez Gene: Syde2 (a.k.a. C430017H16Rik)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV Forward (5'-CGCAAATGGGCGGTAGGCGTG-3')
• 3′ sequencing primer: pNICE_rev_seq (5'-AAGCAAGTAAAACCTCTACAAATG-3'); SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')

Resource Information

• Supplemental Documents:
  • pNICE HA-mSYD1B_sequence file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mSYD1B can be released without HA-tag (by XhoI / BamHI) or with HA-tag (e.g. by AgeI / BamHI).