pLenLox_U6 p53
(Plasmid #59360)

• Purpose: Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.
• Depositing Lab: Peter Scheiffele
• Publication: Chih et al Science. 2005 Feb 25;307(5713):1324-8. Epub 2005 Jan 27.

Backbone

• Vector backbone: pLL3.7
• Backbone size w/o insert (bp): 8777
• Total vector size (bp): 8831
• Modifications to backbone: GFP expression is driven by CAG-promoter (instead of CMV-promoter)
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: Growth in standard bacterial strains (e.g. DH5alpha) at 37°C is possible, however, growth in SURE cells (37°C) or Stbl3 cells (30°C) is recommended to reduce the risk of recombination.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: shRNA p53 (mouse)
• Alt name: p53
• Alt name: Trp53
• Species: M. musculus (mouse)
• Insert Size (bp): 54
• GenBank ID: NM_011640 NM_001127233
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
• Promoter: mouse U6

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: mU6-F (5'-CAGCACAAAAGGAAACTCACC-3'); mU6-F2 (5'-ACAGACTTGTGGGAGAAGC-3')

Gene/Insert 2

• Gene/Insert name: eGFP
• Alt name: enhanced green fluorescent protein
• Insert Size (bp): 720
• Promoter: CAG

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')

Resource Information

• Supplemental Documents:
  • pLenLox_U6 p53_sequence file
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry