pBrain-GFP-TACC3KDP(S558A)-shTACC3
(Plasmid #59357)

• Purpose: Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
• Depositing Lab: Stephen Royle
• Publication: Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10.1038/emboj.2011.15. Epub 2011 Feb 4.

Backbone

• Vector backbone: pBrain
• Backbone manufacturer: Stephen J Royle
• Backbone size w/o insert (bp): 5438
• Total vector size (bp): 7952
• Modifications to backbone: Addition of N-terminal GFP tag. shRNA to knock down endogenous TACC3.
• Vector type: Mammalian Expression, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Transforming Acidic Coiled Coil protein 3
• Alt name: TACC3
• Alt name: ERIC1
• Species: H. sapiens (human)
• Insert Size (bp): 2514
• Mutation: Silent mutations in amino acids 30-33 to confer siRNA resistance. Serine 558 changed to Alanine.
• GenBank ID: NM_006342.2
• Entrez Gene: TACC3 (a.k.a. ERIC-1, ERIC1, Tacc4, maskin)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: shRNA sequence as referenced in Gergely, 2003.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry