pBrain-GFP-FKBP-TACC3-shTACC3
(Plasmid #59354)

• Purpose: Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.
• Depositing Lab: Stephen Royle
• Publication: Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13. doi: 10.1242/jcs.124834. Epub 2013 Mar 26.

Backbone

• Vector backbone: pBrain
• Backbone manufacturer: Stephen J Royle
• Backbone size w/o insert (bp): 5816
• Total vector size (bp): 8330
• Modifications to backbone: Includes EGFP and FKBP to tag protein of interest at N-terminus.
• Vector type: Mammalian Expression, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Transforming Acidic Coiled Coil protein 3
• Alt name: TACC3
• Alt name: ERIC1
• Species: H. sapiens (human)
• Insert Size (bp): 2514
• GenBank ID: NM_006342.2
• Entrez Gene: TACC3 (a.k.a. ERIC-1, ERIC1, Tacc4, maskin)
• Promoter: CMV
• Tags / Fusion Proteins:
  • EGFP (N terminal on backbone)
  • FKBP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TACC3: IMAGE 6148176
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry