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Addgene

pET21a(+)-PA_PhoU
(Plasmid #59334)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59334 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    phoU
  • Alt name
    PA5365
  • Alt name
    phoU
  • Species
    Pseudomonas aeruginosa PAO1
  • Insert Size (bp)
    726
  • GenBank ID
    NC_002516.2
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (CATATG) (destroyed during cloning)
  • 3′ cloning site XhoI (CTCGAG) (destroyed during cloning)
  • 5′ sequencing primer GGAATTCCATATGATGATCAACAAAGACAGTCTC
  • 3′ sequencing primer CCGCCGCTCGAGTTATCACTCGCCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The PhoU ORF has a K139R mutation. This change may be due to an error in the genomic sequencing data. The K139R residue is located on the surface of PhoU protein and the side chain is somewhat disordered, making it difficult to confirm its identity. The mutation should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a(+)-PA_PhoU was a gift from SeWon Suh (Addgene plasmid # 59334 ; http://n2t.net/addgene:59334 ; RRID:Addgene_59334)
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