pAAV-CAG-FLEX-splitTVA-EGFP
(Plasmid
#59332)
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PurposeExpresses splitTVA-P2A-EGFP
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 59332 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 5688
- Total vector size (bp) 6951
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3 at 37C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesplitTVA950-P2A-EGFP
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SpeciesSynthetic
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Insert Size (bp)1263
- Promoter CAG
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer tgctaaccatgttcatgc
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alternative name: pAAV-CAG-FLEX-sTpE
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-CAG-FLEX-splitTVA-EGFP was a gift from Ian Wickersham (Addgene plasmid # 59332 ; http://n2t.net/addgene:59332 ; RRID:Addgene_59332)