pFCcGi
(Plasmid #59324)

• Purpose: Inducible expression of GFP and constitutive expression of mCherry
• Depositing Labs: Sophie Helaine, David Holden
• Publication: Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1128/mBio.00065-13.

Backbone

• Vector backbone: pFPV25.1
• Backbone manufacturer: Addgene Plasmid #20668
• Backbone size w/o insert (bp): 4764
• Total vector size (bp): 7200
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: No special growth conditions are required for optimal induction
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: mCherry
• GenBank ID:
• Promoter: rpsM

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: mCherry-R
• 3′ sequencing primer: PBAD-R

Gene/Insert 2

• Gene/Insert name: EGFP
• Alt name: gfpmut3a
• Promoter: Pbad

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: PBAD-F

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that a ~2.4kb band may appear in restriction digest experiments which represents single-stranded DNA (ssDNA).