pDiGi
(Plasmid #59323)

• Purpose: Inducible expression of dsRed and GFP in bacteria
• Depositing Labs: Sophie Helaine, David Holden
• Publication: Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3746-51. doi: 10.1073/pnas.1000041107. Epub 2010 Feb 2.

Backbone

• Vector backbone: pBAD
• Total vector size (bp): 7900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: Bacteria are better induced for dsRed after O/N in minimum medium (MgMES medium is ideal: 170mM 2-[N-morpholino]ethane-sulphonic acid at pH 5.0, 5 mM KCl, 7.5 mM (NH4)2SO4, 0.5 mM K2SO4, 1 mM KH2PO4, 10 mM MgCl2, 38 mM glycerol and 0.1% casamino acids (adapted from Beuzone et al., 1999)) in presence of arabinose (0.2% w/v). GFP induced by IPTG 0.5 mM.
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: EGFP
• Alt name: gpfmut3a
• Promoter: Plac

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Lac-promoter

Gene/Insert 2

• Gene/Insert name: dsRed
• Alt name: DsRed.T3_S4T
• Promoter: Pbad

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: PBAD-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry