pCAG-HA-NLGN3 R451C
(Plasmid #59319)

• Purpose: Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation
• Depositing Lab: Peter Scheiffele
• Publication: Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7. Epub 2004 May 18.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4835
• Total vector size (bp): 7313
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NLGN3 R451C
• Alt name: Neuroligin 3
• Species: H. sapiens (human)
• Insert Size (bp): 2478
• Mutation: Arginine 451 to Cysteine (R451C mutation)
• GenBank ID: NM_018977 BC051715
• Entrez Gene: NLGN3 (a.k.a. HNL3)
• Promoter: CAG
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
• 3′ sequencing primer: Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')

Resource Information

• Supplemental Documents:
  • pCAG-HA-NLGN3 R451C_sequence file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / XhoI).
HA-epitope tag was introduced at the mature N-terminus after the signal sequence