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Addgene

pNice-HA-alpha2-chimaerin
(Plasmid #59315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNICE-HA-N
  • Backbone size w/o insert (bp) 3971
  • Total vector size (bp) 5387
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha2-chimaerin
  • Alt name
    a2-chimaerin
  • Insert Size (bp)
    1416
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CMV Forward (5'-CGCAAATGGGCGGTAGGCGTG-3')
  • 3′ sequencing primer pNICE_rev_seq (5'-AAGCAAGTAAAACCTCTACAAATG-3'); SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See map & sequence file for insert-releasing enzymes.
alpha2-chimaerin can be released without HA-tag (by SalI / NotI) or with HA-tag (e.g. by EcoRI / NotI).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNice-HA-alpha2-chimaerin was a gift from Peter Scheiffele (Addgene plasmid # 59315 ; http://n2t.net/addgene:59315 ; RRID:Addgene_59315)

  • For your References section:

    alpha2-Chimaerin is an essential EphA4 effector in the assembly of neuronal locomotor circuits. Beg AA, Sommer JE, Martin JH, Scheiffele P. Neuron. 2007 Sep 6;55(5):768-78. 10.1016/j.neuron.2007.07.036 PubMed 17785183
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