pcDNA3-Nrxn1beta AS4(+)-Fc
(Plasmid #59314)

• Purpose: Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
• Depositing Labs: Peter Scheiffele, Tito Serafini
• Publication: Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 6672
• Total vector size (bp): 7545
• Modifications to backbone: Fc-tag (from human IgG) added, resulting in secreted Fc-fusion protein
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nrxn1beta AS4(+)-Fc fusion
• Alt name: Nrxn1beta AS4(+)
• Alt name: Neurexin 1 beta AS4(+)
• Alt name: Neurexin 1 beta (splice 4)
• Species: M. musculus (mouse)
• Insert Size (bp): 873
• GenBank ID:
• Entrez Gene: Nrxn1 (a.k.a. 1700062G21Rik, 9330127H16Rik, A230068P09Rik, mKIAA0578)
• Promoter: CMV, T7
• Tag / Fusion Protein:
  • Fc (from human IgG) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: CMV Forward (5'-CGCAAATGGGCGGTAGGCGTG-3'), T7 (5'-TAATACGACTCACTATAGGG-3')
• 3′ sequencing primer: FC-rev (5'-CGGGTGAAGAGGCAGACG-3'); SP6 (5'-ATTTAGGTGACACTATAG-3')

Resource Information

• Supplemental Documents:
  • pcDNA3-Nrxn1beta AS4(+)-Fc_sequence file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Fc-portion contains intronic sequences which improve expression.
See map & sequence file for insert-releasing enzymes (e.g. HindIII / BglII for untagged Nrxn1beta, and HindIII / XhoI for Nrxn1beta-FC-fusion).
The Nrxn 1 beta variant encoded by this plasmid is AS4(+), meaning exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is present.