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Addgene

pLL3.7 Lmo3.5 shRNA
(Plasmid #59295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59295 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.7
  • Backbone manufacturer
    Addgene Plasmid #11795
  • Backbone size w/o insert (bp) 7650
  • Total vector size (bp) 7579
  • Vector type
    Mammalian Expression, Lentiviral, RNAi, Cre/Lox
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Although this plasmid grows in standard bacteria, to reduce the chance of recombination we recommend that you grow the plasmid at 30oC in Stbl3 cells from Invitrogen.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lmo3.5 shRNA
  • Alt name
    Lmo3.5
  • gRNA/shRNA sequence
    GAGCAAACCTCCACAATGA
  • Species
    M. musculus (mouse)
  • Promoter mouse U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer U6 (5'-cagtgcaggggaaagaatagtagac-3')
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Luk Parijs

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL3.7 Lmo3.5 shRNA was a gift from Ulrike Heberlein (Addgene plasmid # 59295 ; http://n2t.net/addgene:59295 ; RRID:Addgene_59295)

  • For your References section:

    Lmo genes regulate behavioral responses to ethanol in Drosophila melanogaster and the mouse. Lasek AW, Giorgetti F, Berger KH, Tayor S, Heberlein U. Alcohol Clin Exp Res. 2011 Sep;35(9):1600-6. doi: 10.1111/j.1530-0277.2011.01506.x. Epub 2011 May 20. 10.1111/j.1530-0277.2011.01506.x PubMed 21599714
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