pLL3.7 Lmo4.2 shRNA
(Plasmid #59293)

• Purpose: shRNA to mouse Lmo4
• Depositing Lab: Ulrike Heberlein
• Publication: Lasek et al Genes Brain Behav. 2010 Oct;9(7):817-24. doi: 10.1111/j.1601-183X.2010.00620.x. Epub 2010 Aug 12.

Backbone

• Vector backbone: pLL3.7
• Backbone manufacturer: Addgene Plasmid #11795
• Backbone size w/o insert (bp): 7650
• Total vector size (bp): 7579
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: Although this plasmid grows in standard bacteria, to reduce the chance of recombination we recommend that you grow the plasmid at 30oC in Stbl3 cells from Invitrogen.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lmo4.2 shRNA
• Alt name: Lmo4.2
• gRNA/shRNA sequence: GAGCGGCATGATCCTTTGC
• Species: M. musculus (mouse)
• Promoter: mouse U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: U6 (5'-cagtgcaggggaaagaatagtagac-3')

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Luk Parijs

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression. Supported by the NIAAA INIA West consortium.