pDam-Myc-HP1
(Plasmid #59221)

• Purpose: expresses Dam-myc-HP1
• Depositing Lab: Bas van Steensel
• Publication: van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Backbone

• Vector backbone: pNDam-myc, Addgene plasmid 59217
• Backbone manufacturer: van Steensal lab
• Total vector size (bp): 10271
• Vector type: Insect Expression ; DamID

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HP1
• Alt name: Suppressor of variegation 205
• Alt name: Su(var)205
• Alt name: heterochromatin protein 1
• Species: D. melanogaster (fly)
• Entrez Gene: Su(var)205 (a.k.a. Dmel_CG8409, CBX5, CG8409, DmHP-1, DmHP1, Dmel\CG8409, E(var)29, E(var)29A, FBgn0003607, HP-1, HP1, HP1-VS, HP1A, HP1a, HP1alpha, Hp1, Hp1a, SU(VAR)205, Su(Var)2-5, Su(Var)205, Su(var), Su(var)2-05, Su(var)2-5, Su(var)2-501, Su(var)29A, Su(var)[205], Su-var(2)5, Su[Var]205, Su[var]205, Sur(var)205, Suvar(2)5, Suvar2-5, dHP-1a, dHP1, dHP1a, hp1, hp1a, su(var)205)
• Promoter: Drosophila heat shock promoter
• Tags / Fusion Proteins:
  • Dam (N terminal on backbone)
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pCasper-hs

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry