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pLgw Cbx1-V5-EcoDam
(Plasmid #59211)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59211 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HIV-CS-CG
  • Backbone manufacturer
    PMID 9733856
  • Vector type
    Mammalian Expression, Lentiviral ; DamID
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cbx1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cbx1 (a.k.a. Cbx, Cbx-rs2, E430007M08Rik, HP1B, Hp1beta, M31, MOD1)
  • Promoter heat shock minimal pomoter
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • Dam (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector may be used as a positive control. It expresses the Cbx1-V5-Dam protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLgw Cbx1-V5-EcoDam was a gift from Bas van Steensel (Addgene plasmid # 59211 ; http://n2t.net/addgene:59211 ; RRID:Addgene_59211)

  • For your References section:

    Human heterochromatin proteins form large domains containing KRAB-ZNF genes. Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, Feenstra M, Abbas B, Classen AK, van Steensel B. Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12. 10.1101/gr.5391806 PubMed 17038565
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