pLgw V5-EcoDam
(Plasmid #59210)

• Purpose: Mammalian DamID control lentiviral vector for expression of V5-Dam only
• Depositing Lab: Bas van Steensel
• Publication: Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.

Backbone

• Vector backbone: HIV-CS-CG
• Backbone manufacturer: PMID 9733856
• Vector type: Mammalian Expression, Lentiviral ; DamID
• Selectable markers: Zeo marker is outside the LTRs and will not be packaged into virus.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EcoDam
• Alt name: DNA adenine methyltransferase
• Species: E. coli
• Promoter: heat shock minimal pomoter
• Tag / Fusion Protein:
  • V5 (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCasper-hs

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is the control vector for expression of V5-Dam only (the absolutely essential control experiment!).