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Addgene

pINDgw Cbx1 V5 EcoDam
(Plasmid #59203)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 59203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pINDgw RFA-V5-EcoDam
  • Backbone manufacturer
    van Steensel lab, Addgene plasmid 59204
  • Total vector size (bp) 6596
  • Vector type
    Mammalian Expression ; DamID
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cbx1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cbx1 (a.k.a. Cbx, Cbx-rs2, E430007M08Rik, HP1B, Hp1beta, M31, MOD1)
  • Promoter heat shock minimal pomoter
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • Dam (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pINDgw Cbx1 V5 EcoDam was a gift from Bas van Steensel (Addgene plasmid # 59203 ; http://n2t.net/addgene:59203 ; RRID:Addgene_59203)

  • For your References section:

    Human heterochromatin proteins form large domains containing KRAB-ZNF genes. Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, Feenstra M, Abbas B, Classen AK, van Steensel B. Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12. 10.1101/gr.5391806 PubMed 17038565
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