pBlu/gRNA
(Plasmid #59188)

• Purpose: Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector
• Depositing Lab: Robert Stupar
• Publication: Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.

Backbone

• Vector backbone: pBluescript KS(+)
• Backbone size w/o insert (bp): 2958
• Total vector size (bp): 3515
• Modifications to backbone: Added a gRNA cassette with an Arabidopsis U6 promoter and terminator
• Vector type: Plant Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Guide RNA Cassette
• Alt name: gRNA
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 551
• Promoter: U6 (Arabidopis)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T5

Resource Information

• Supplemental Documents:
  • Stupar Lab Protocol
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry