G10 Cas9 MDC123
(Plasmid #59187)

• Purpose: G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection
• Depositing Lab: Robert Stupar
• Publication: Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.

Backbone

• Vector backbone: pMDC123
• Backbone manufacturer: Mark D Curtis
• Backbone size w/o insert (bp): 10213
• Total vector size (bp): 13191
• Modifications to backbone: Added G10 promoter instead of the 2X35S promoter and added a Glycine Max Codon Optimized Cas9 with an N and C terminus nuclear localization signal.
• Vector type: Plant Expression, CRISPR
• Selectable markers: Basta

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: It may take longer to grow than other plasmids due to the Cas9
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Glycine Max codon optimized Cas9
• Alt name: Cas9
• Species: Glycine Max
• Insert Size (bp): 4140
• Promoter: G10

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: M13_forward20_primer
• 3′ sequencing primer: M13_reverse_primer

Resource Information

• Supplemental Documents:
  • Stupar Lab Protocol

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry