pMXs-hFOXH1
(Plasmid
#59183)
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PurposeForced expression of human FOXH1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 59183 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMXs-gw
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Backbone manufacturerYamanaka Lab
- Backbone size w/o insert (bp) 4600
- Total vector size (bp) 5700
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFOXH1
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Alt nameForkhead box H1
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SpeciesH. sapiens (human)
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Insert Size (bp)1100
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GenBank IDNM_003923
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Entrez GeneFOXH1 (a.k.a. FAST-1, FAST1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pMX-S1811 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGateway cassette was purchased from Invitrogen.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-hFOXH1 was a gift from Shinya Yamanaka (Addgene plasmid # 59183 ; http://n2t.net/addgene:59183 ; RRID:Addgene_59183) -
For your References section:
Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm. Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S. Nat Commun. 2014 Apr 24;5:3678. doi: 10.1038/ncomms4678. 10.1038/ncomms4678 PubMed 24759836