pLX304-YAP1 _60-89
(Plasmid #59144)

• Purpose: YAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factors
• Depositing Lab: William Hahn
• Publication: Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.1016/j.cell.2014.06.004. Epub 2014 Jun 19.

Backbone

• Vector backbone: pLX304
• Backbone manufacturer: Addgene plasmid 25890
• Total vector size (bp): 9377
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YAP1
• Species: H. sapiens (human)
• Mutation: aa60-89 deleted
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry