pOTTC592 - pCMV Mito-iRFP
(Plasmid #59135)

• Purpose: A plasmid that express mitochondrial-targeted iRFP under the CMV promoter.
• Depositing Lab: Brandon Harvey
• Publication: Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.

Backbone

• Vector backbone: pDsRed-Mito
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mitochondria-localized Infrared Fluorescent Protein
• Alt name: iRFP713
• Promoter: CMV-IE
• Tag / Fusion Protein:
  • Mitochondria-targeting sequence (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV F
• 3′ sequencing primer: SV40pA R

Resource Information

• Supplemental Documents:
  • pOTTC592 - pCMV-IE Mito-iRFP.ape.ape
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry