pCS2-FAK20
(Plasmid #59130)

• Purpose: Cloning vector for FAK fret sensor with 20 nm helix
• Depositing Lab: Sivaraj Sivaramakrishnan
• Publication: Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86. doi: 10.1074/jbc.M112.421164. Epub 2013 Feb 7.

Backbone

• Vector backbone: pcDNA-5/FRT
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3777
• Total vector size (bp): 9004
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Focal Adhesion Kinase
• Alt name: FAK
• Species: G. gallus (chicken), Synthetic
• Insert Size (bp): 5227
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • mCitrine (N terminal on insert)
  • mCerulean (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHR

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry