pAAV-Syn-CoChR-GFP
(Plasmid #59070)

• Purpose: AAV-mediated expression of CoChR-GFP under the Syn promoter
• Depositing Lab: Edward Boyden
• Publication: Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9.

Backbone

• Vector backbone: AAV
• Backbone size w/o insert (bp): 4368
• Total vector size (bp): 5967
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C (use ampicillin if using DH5a)
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CoChR-GFP
• Alt name: ChR86-GFP
• Alt name: Chloromonas oogama channelrhodopsin
• Species: Chloromonas oogama
• Insert Size (bp): 1599
• GenBank ID: KF992041
• Promoter: Syn
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CTGCGTATGAGTGCAAG
• 3′ sequencing primer: cagcgtatccacatagcg

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is completely sequenced by the depositing lab except for part of the 3' ITR. Addgene QC NGS analysis identifies ambiguous bases following the 3' ITR. It is unknown what, if any, impact this may have on plasmid function.