FCK-ChrimsonR-GFP
(Plasmid #59049)

• Purpose: Lentiviral expression of ChrimsonR under the CaMKII promoter
• Depositing Lab: Edward Boyden
• Publication: Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 2014 Feb 9.

Backbone

• Vector backbone: FCK(1.3)GW
• Backbone manufacturer: Pavel Osten
• Backbone size w/o insert (bp): 9238
• Total vector size (bp): 11023
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ChrimsonR-GFP
• Alt name: Chrimson K176R mutant
• Alt name: ChR88m19-GFP
• Species: Chlamydomonas noctigama
• Insert Size (bp): 1785
• Mutation: K176R
• GenBank ID: KF992060
• Promoter: CaMKII
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TCTCCATTTGCACTCAGG
• 3′ sequencing primer: CAGCGTATCCACATAGCG

Resource Information

• Supplemental Documents:
  • Annotated GenBank File
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has been fully sequenced by the depositing lab.

Please see the plasmid map and GenBank file for custom annotations by the depositing lab.