pcdna3 Flag Jip2
(Plasmid #59027)

• Purpose: act as molecular scaffolds that mediate the activation of the JNK signaling pathway in vivo.
• Depositing Lab: Roger Davis
• Publication: Yasuda et al Mol Cell Biol. 1999 Oct;19(10):7245-54.

Backbone

• Vector backbone: pcdna3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 7950
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitogen-activated protein kinase 8 interacting protein 2
• Alt name: MAPK8IP2
• Alt name: Jip2
• Insert Size (bp): 2504
• GenBank ID: AF136382
• Entrez Gene: MAPK8IP2 (a.k.a. IB-2, IB2, JIP2, PRKM8IPL)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry