pGex-4T-1 Jip4 (1-272)
(Plasmid #59002)

• Purpose: JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6
• Depositing Lab: Roger Davis
• Publication: Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.

Backbone

• Vector backbone: pGex-4T-1
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 5610
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mapk8ip4
• Alt name: Jip4
• Species: M. musculus (mouse)
• Insert Size (bp): 710
• Mutation: Jip4 (aa1-272)
• GenBank ID: AY823270.1
• Entrez Gene: Spag9 (a.k.a. 3110018C07Rik, 4733401I23Rik, 4831406C20Rik, JIP-4, JLP, JSAP2, JSAP2a, Jip4, Mapk8ip4, syd1)
• Tag / Fusion Protein:
  • Gst (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: Gex 5'
• 3′ sequencing primer: Gex 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry