pTNT-B18R-6His
(Plasmid #58979)

• Purpose: expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs
• Depositing Lab: Steven Dowdy
• Publication: Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001.

Backbone

• Vector backbone: pTNT
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2871
• Total vector size (bp): 3948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: B18R
• Alt name: ankyrin repeat protein
• Species: Vaccinia Virus
• Promoter: T7/Sp6
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MfeI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

B18R from Addgene plasmid #12794