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T7-VEE-OKS-iM
(Plasmid #58972)

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Item Catalog # Description Quantity Price (USD)
Plasmid 58972 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    T7-VEE-IRES-puro (Addgene plasmid 58970)
  • Backbone manufacturer
    Dowdy lab
  • Vector type
    Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Oct4
  • Species
    H. sapiens (human)
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
  • Promoter 26S subgenomic promoter

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Klf4
  • Alt name
    Kruppel-like factor 4
  • Species
    H. sapiens (human)
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 3

  • Gene/Insert name
    Sox2
  • Alt name
    SRY (sex determining region Y)-box 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Gene/Insert 4

  • Gene/Insert name
    cMyc
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter IRES

Cloning Information for Gene/Insert 4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Summary of features:
1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter, nsP1~4 are nonstructural VEE sequences which encode RNA replicases)
7592-8671: human Oct4
8678-8731: T2A peptide sequence
8738-10147: human Klf4
10154-10213: E2A peptide sequence
10223-11176: human Sox2
11195-11805: IRES sequence
11818-13140: human cMyc
13165-13776: IRES sequence
13777-14376: Puromycin resistance gene
14383-14510:VEE 3’UTR and poly A tail
14679-15539: Ampicillin resistance gene
16319-16336: T7 promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-VEE-OKS-iM was a gift from Steven Dowdy (Addgene plasmid # 58972 ; http://n2t.net/addgene:58972 ; RRID:Addgene_58972)

  • For your References section:

    Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086
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