pJC30X-BS(X)
(Plasmid #58958)

• Purpose: Encodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
• Depositing Lab: Thomas Fox
• Publication: Cohen et al Mitochondrion. 2001 Aug;1(2):181-9.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 8300

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Enhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 5400
• Mutation: GFP modified as described: CORMACK, B. P., R. H. VALDIVIA and S. FALKOW, 1996 FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
• GenBank ID: U89686.1
• Promoter: n/a
• Tag / Fusion Protein:
  • First 8 codons of COX3 (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry