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Addgene

pDS24
(Plasmid #58952)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 8050

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mitochondrially coded Arg8 protein
  • Alt name
    acetylornithine aminotransferase
  • Alt name
    ARG8m
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2042
  • Mutation
    Coding sequence synthetic in S. cerevisiae mitochondrial genetic code
  • GenBank ID
    U31093.1
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Insert also contains an internal SacI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDS24 was a gift from Thomas Fox (Addgene plasmid # 58952 ; http://n2t.net/addgene:58952 ; RRID:Addgene_58952)

  • For your References section:

    Expression of a recoded nuclear gene inserted into yeast mitochondrial DNA is limited by mRNA-specific translational activation. Steele DF, Butler CA, Fox TD. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5253-7. PubMed 8643562
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