pVk7-33G1-BioLHis
(Plasmid #58949)

• Purpose: (Empty Backbone) backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag
• Depositing Lab: Gavin Wright
• Publication: Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. doi: 10.1016/j.bbrc.2014.11.123. Epub 2014 Dec 6.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size (bp): 5925
• Modifications to backbone: Kozak and leader of Vkappa 7-33, Gamma1 heavy constant region, C-terminal addition of monobiotinylation site, linker sequence and 6His
• Vector type: Mammalian Expression
• Promoter: CMV
• Tags / Fusion Proteins:
  • biotinylation peptide (C terminal on backbone)
  • 6His tag (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry