SI16-Kit a
(Plasmid #58948)

• Purpose: expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells
• Depositing Lab: Gavin Wright
• Publication: Crosnier et al BMC Biol. 2010 . 8():76.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 5925
• Total vector size (bp): 8350
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Kit receptor a mouse monoclonal antibody
• Alt name: kita
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2400
• Entrez Gene: kita (a.k.a. c-kit, kit, receptor, sparse)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Asc1 (not destroyed)
• 5′ sequencing primer: M13-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada - Biotechnology Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry