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Addgene

pEBG Jip3 (nterm)
(Plasmid #58942)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBG
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 6810
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitogen-activated protein kinase 8 interacting protein 3
  • Alt name
    Jip3
  • Alt name
    Mapk8ip3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    810
  • Mutation
    deleted aa 271-1817
  • GenBank ID
    AF178636
  • Entrez Gene
    Mapk8ip3 (a.k.a. D17Wsu15e, JIP-3, JSAP1, JSAP1a, JSAP1b, JSAP1c, JSAP1d, Jip3, Syd2, mKIAA1066)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer Gex 5'
  • 3′ sequencing primer Gex 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBG Jip3 (nterm) was a gift from Roger Davis (Addgene plasmid # 58942 ; http://n2t.net/addgene:58942 ; RRID:Addgene_58942)

  • For your References section:

    Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3. Kelkar N, Gupta S, Dickens M, Davis RJ. Mol Cell Biol. 2000 Feb;20(3):1030-43. PubMed 10629060
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