Skip to main content
Addgene

pcdna3 Flag Jip3b(T266A, T276A, T287A)
(Plasmid #58924)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcdna3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 11008
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitogen-activated protein kinase 8 interacting protein 3
  • Alt name
    Jip3b(T266A, T276A, T287A)
  • Alt name
    Mapk8ip3-001
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5562
  • Mutation
    changed T266A, T276A, T287A
  • GenBank ID
  • Entrez Gene
    Mapk8ip3 (a.k.a. D17Wsu15e, JIP-3, JSAP1, JSAP1a, JSAP1b, JSAP1c, JSAP1d, Jip3, Syd2, mKIAA1066)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3 Flag Jip3b(T266A, T276A, T287A) was a gift from Roger Davis (Addgene plasmid # 58924 ; http://n2t.net/addgene:58924 ; RRID:Addgene_58924)

  • For your References section:

    Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3. Kelkar N, Gupta S, Dickens M, Davis RJ. Mol Cell Biol. 2000 Feb;20(3):1030-43. PubMed 10629060
Related items:
Commonly requested with: