pCMV HA hRB delta CDK + S612
(Plasmid #58914)

• Purpose: mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored
• Depositing Lab: Steven Dowdy
• Publication: Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/eLife.02872.

Backbone

• Vector backbone: pCMV
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rb
• Alt name: retinoblastoma tumor suppressor protein
• Species: H. sapiens (human)
• Insert Size (bp): 2787
• Mutation: delta CDK* with S612 restored
• Entrez Gene: RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*CDK mutations (all changed to Ala): T5, S239, S249, T252, T356, T373, S608, S612, S780, S788, S795, S807, S811, T821, T826