piCRg Entry
(Plasmid #58904)

• Purpose: Cas9/gRNA Entry expression plasmid
• Depositing Lab: Danwei Huangfu
• Publication: Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018.

Backbone

• Vector backbone: Low copy
• Backbone manufacturer: home made
• Backbone size w/o insert (bp): 2570
• Total vector size (bp): 8302
• Modifications to backbone: inroduction of the pBR322 (low copy) origin of replication
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hSpCas9
• Insert Size (bp): 4101

Resource Information

• Supplemental Documents:
  • piCRg Entry Genbank file
• A portion of this plasmid was derived from a plasmid made by: The human codon-optimized S. pyogenes Cas9 was amplified by PCR from plasmid pX260 (Cong et al.,Science. 2013). The chimeric U6-gRNA expression cassette was PCR amplified from pX330 (Cong et al.,Science. 2013).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry