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Addgene

pBabe-Kras G12V-T35S
(Plasmid #58900)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBabe-puro
  • Backbone manufacturer
    unknown
  • Backbone size w/o insert (bp) 5169
  • Total vector size (bp) 5906
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KRAS
  • Alt name
    K-Ras2A
  • Alt name
    RASK2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    G12V and T35S mutations (effector binding mutant)
  • GenBank ID
    NM_004985.4 NP_004976.2
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Kras G12V-T35S was a gift from Channing Der (Addgene plasmid # 58900 ; http://n2t.net/addgene:58900 ; RRID:Addgene_58900)
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