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PurposeExpresses human Kras with a S17N mutation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe-puro
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Backbone manufacturerunknown
- Backbone size w/o insert (bp) 5169
- Total vector size (bp) 5906
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKRAS
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Alt nameK-Ras2A
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Alt nameRASK2
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SpeciesH. sapiens (human)
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Insert Size (bp)600
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MutationS17N mutation
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GenBank IDNM_004985.4 NP_004976.2
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Entrez GeneKRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-Kras S17N was a gift from Channing Der (Addgene plasmid # 58899 ; http://n2t.net/addgene:58899 ; RRID:Addgene_58899)