pEGFP TEM4 R130D
(Plasmid #58895)

• Purpose: Expresses GFP Tagged full length TEM4 protein with R130D mutation. Please see notes below for information regarding the L722F mutation in TEM4.
• Depositing Lab: Channing Der
• Publication: Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371/journal.pone.0041876. Epub 2012 Jul 24.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 10900
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TEM4
• Alt name: ARHGEF17
• Alt name: P164RHOGEF
• Species: H. sapiens (human)
• Insert Size (bp): 6190
• Mutation: R130D, L722F
• GenBank ID: NM_014786.3 NP_055601.2
• Entrez Gene: ARHGEF17 (a.k.a. P164RHOGEF, TEM4, p164-RhoGEF)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
• 3′ sequencing primer: SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence identified a L722F mutation in TEM4. It is unknown whether this mutation affects protein function.