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Addgene

pEGFP TEM4 DEL NTERM
(Plasmid #58894)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 58894 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7880
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TEM4
  • Alt name
    ARHGEF17
  • Alt name
    P164RHOGEF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3180
  • Mutation
    Deleted N Terminus up to aa 1002
  • GenBank ID
    NM_014786.3 NP_055601.2
  • Entrez Gene
    ARHGEF17 (a.k.a. P164RHOGEF, TEM4, p164-RhoGEF)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
  • 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP TEM4 DEL NTERM was a gift from Channing Der (Addgene plasmid # 58894 ; http://n2t.net/addgene:58894 ; RRID:Addgene_58894)

  • For your References section:

    Identification of a novel actin-binding domain within the Rho guanine nucleotide exchange factor TEM4. Mitin N, Rossman KL, Der CJ. PLoS One. 2012;7(7):e41876. doi: 10.1371/journal.pone.0041876. Epub 2012 Jul 24. 10.1371/journal.pone.0041876 PubMed 22911862
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