pAAV-GFAP104-ChR2-mCherry
(Plasmid #58892)

• Purpose: AAV-mediated expression of ChR2-mCherry under the GFAP promoter
• Depositing Lab: Edward Boyden
• Publication: Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/ncomms4262.

Backbone

• Vector backbone: AAV
• Backbone size w/o insert (bp): 4895
• Total vector size (bp): 6545
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ChR2-mCherry
• Alt name: channelrhodopsin-2-mCherry
• Species: Chlamydomonas reinhardtii
• Insert Size (bp): 1650
• GenBank ID: EU714030.1
• Promoter: GFAP104
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ggagagctctccccatag
• 3′ sequencing primer: cagcgtatccacatagcg

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The depositing lab has sequenced the 5' ITR, promoter, and gene-fluorophore. Multiple digestions were done to verify the vector structure. The construct was tested in vitro and the virus was tested both in vitro and in vivo.