p5'Entry-lck-5.5kb
(Plasmid #58890)

• Purpose: provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes
• Depositing Lab: Jeffrey Yoder
• Publication: Zebrafish lymphocyte-specific protein tyrosine kinase (lck) 5' entry clones (unpublished)

Backbone

• Vector backbone: pDONR P4-P1R
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4777
• Total vector size (bp): 8156
• Vector type: Gateway 5' entry clone

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
• Species: D. rerio (zebrafish)
• Insert Size (bp): 5513
• Mutation: mutated single nucleotide (T-->C, 13th nucleotide of insert) to create novel SfiI site at 5' end of promoter
• Promoter: lymphocyte-specific protein tyrosine kinase (lck), 5.5 kb

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • Cloning of zebrafish 5.5 kb promoter
• A portion of this plasmid was derived from a plasmid made by: Zebrafish lck promoter was PCR amplified using BAC clone CH211-135B6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry