pAAV-CaMKII-Mac-GFP
(Plasmid #58849)

• Purpose: AAV expression of Mac-GFP under the CaMKII promoter
• Depositing Lab: Edward Boyden
• Publication: Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Backbone

• Vector backbone: AAV
• Backbone size w/o insert (bp): 5087
• Total vector size (bp): 6758
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Mac-GFP
• Alt name: Leptosphaeria maculans opsin
• Species: Leptosphaeria maculans
• Insert Size (bp): 1671
• GenBank ID: GU045595.1
• Promoter: CaMKII
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: tgctaaccatgttcatgc
• 3′ sequencing primer: cagcgtatccacatagcg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is sequenced completely by the depositing lab except for parts of both ITRs. There are around 10 traditional basepair differences in the CaMKII promoter relative to the wild-type, which is not a problem. Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.