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PurposeExpresses GFP-Tagged OSBP PH domain in yeast
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58840 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS406
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOxysterol-binding Protein PH domain
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Alt nameOSBP1
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SpeciesH. sapiens (human)
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GenBank IDNM_002556.2
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Entrez GeneOSBP (a.k.a. OSBP1)
- Promoter PHO5
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PH domain of OSBP= Serine 87 - Lysine 185
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS406-PHO5-GFP-OSBP PH domain was a gift from Tim Levine & Sean Munro (Addgene plasmid # 58840 ; http://n2t.net/addgene:58840 ; RRID:Addgene_58840) -
For your References section:
The pleckstrin homology domain of oxysterol-binding protein recognises a determinant specific to Golgi membranes. Levine TP, Munro S. Curr Biol. 1998 Jun 18;8(13):729-39. S0960-9822(98)70296-9 [pii] PubMed 9651677