pRS416-PHO5-GFP-Osh3
(Plasmid
#58839)
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PurposeExpresses GFP-Tagged Osh3 in yeast
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58839 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS416
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Backbone manufacturerStratagene
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOsh3p
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Alt nameYHR073W
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SpeciesS. cerevisiae (budding yeast)
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GenBank IDNM_001179203.1
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Entrez GeneOSH3 (a.k.a. YHR073W)
- Promoter PHO5
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Tags
/ Fusion Proteins
- GFP (N terminal on backbone)
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS416-PHO5-GFP-Osh3 was a gift from Tim Levine & Sean Munro (Addgene plasmid # 58839 ; http://n2t.net/addgene:58839 ; RRID:Addgene_58839) -
For your References section:
Dual targeting of Osh1p, a yeast homologue of oxysterol-binding protein, to both the Golgi and the nucleus-vacuole junction. Levine TP, Munro S. Mol Biol Cell. 2001 Jun;12(6):1633-44. 10.1091/mbc.12.6.1633 PubMed 11408574